The effects of protein dose response on myofibrillar and mitochondrial protein synthesis after endurance exercise in young men.

Completed

• Conditions: Muscle anabolism; muscle protein deposition; 10037008; 10028302

• Registration Number: NL-OMON42627
• Lead Sponsor: niversiteit Maastricht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 11 June 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 64

Inclusion Criteria

- Males
- Aged between 18-35 years inclusive
- Bodyweight between 60-95 kg inclusive
- Healthy, moderately trained
- BMI < 30 kg/m2
- Having given informed consent

Exclusion Criteria

- Having any identified metabolic or intestinal disorders
- Use of tobacco products
- Non-steroidal anti-inflammatory drugs (NSAID) in the 4 days prior to the experimental trial
- Allergies to milk proteins (whey or casein)
- Arthritic conditions
- A history of neuromuscular problems
- Previous participation in amino acid tracer studies
- Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
- Diabetes
- Training more than 6 days per week
- VO2max < 35 ml/kg/min or > 70 ml/kg/min
- Lactose Intolerance
- Phenylketonuria
- Blood donation within 2 months of study initiation

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Myofibrillar, and mitochondrial protein bound [13C6] phenylalanine and [13C6]<br /><br>leucine enrichments.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Plasma glucose, insulin, leucine, phenylalanine, tyrosine, plasma<br /><br>[13C6]phenylalanine, plasma [13C6] leucine, plasma D5-phenylalanine and plasma<br /><br>(3,5-D2)-tyrosine enrichments.</p><br>