The effect of dietary rapeseed (canola) versus olive oil supplementation on serum lipids, liver enzymes and postprandial inflammatory responses in adipose tissue in obese men.
Completed
- Conditions
- ObesityNutritional, Metabolic, Endocrine
- Registration Number
- ISRCTN22073289
- Lead Sponsor
- The Union for the Promotion of Oil and Protein Plants e.V. (Union zur Foerderung von Oel- und Proteinpflanzen e.V) (Germany)
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Male
- Target Recruitment
- 20
Inclusion Criteria
1. Males, age between 18 and 65 years
2. Obesity, BMI between 27-35 kg/m²
Exclusion Criteria
1. Diabetes mellituis
2. Alcohol or drug abuse
3. Any medical treatment that affects glucose metabolism, treatment with corticosteroids, treatment with monoaminooxidase inhibitors
4. Any treatment with anticoagulants or any known diseases that affect blood clotting
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method To assess the influence of a daily nutritional supplementation with either 50 g of rapeseed oil or 50 g of olive oil to an isocaloric diet on body composition, serum lipids, serum liver enzymes and inflammatory gene expression in sub cutaneous adipose tissue. Body composition will be determined using an air-displacement plethysmography system and serum lipids and liver enzymes will be measured using standard techniques in a certified laboratory at the German Institute of Human Nutrition before and four weeks after dietary intervention. Inflammatory gene expression in sub cutaneous adipose tissue will be measured four weeks after dietary intervention using quantitative real time-PCR.
- Secondary Outcome Measures
Name Time Method To assess the influence of a daily nutritional supplementation with either 50 g of rapeseed oil or 50 g of olive oil to an isocaloric diet on plasma n-6 and n-3 PUFA content and plasma n-6/n-3 ratio before and four weeks after dietary intervention. n-6 and n-3 PUFA content will be determined after lipid extraction from plasma using thin layer chromatography and gas chromatography. The plasma n-6/n-3 ratio will be calculated.