DNA Methylation and 1-C Metabolism Nutrient Intake

Completed

• Conditions: DNA Methylation

• Registration Number: NCT03227003
• Lead Sponsor: Cancer Council Victoria

Brief Summary

The present study used a sub-sample of the Melbourne Collaborative Cohort Study (MCCS) (Milne RL et al, Int J Epidemiol, 2017), who had valid data on dietary intakes (121-item food frequency questionnaire) and valid measures of DNA methylation using the HumanMethylation Infinium 450K Beadchip assay, or using LINE-1, Alu and Sat2 assays.

The investigators studied the association between nutrients involved in one-carbon metabolism and DNA methylation in peripheral blood.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-01-01
• Primary Completion: 2015-12-31
• Study Completion: 2015-12-31
• Status Verified: 2017-07
• Study First Submitted: 2017-07-21
• Study First Submitted QC: 2017-07-21
• Last Update Submitted: 2017-07-21
• Study First Posted: 2017-07-24
• Last Update Posted: 2017-07-24

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 5983

Inclusion Criteria

• Selected into one of six case-control studies nested in the Melbourne Collaborative Cohort Study
• Completed a 121-item Food Frequency Questionnaire
• Had a blood DNA sample with a measure of methylation

Exclusion Criteria

• Missing data in any of the outcome, exposure, or confounders (N=797 excluded)

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Blood DNA methylation	2014-2015	Methylation of DNA from peripheral blood, HumanMethylation 450 Beadchip assay

Secondary Outcome Measures

Name	Time	Method
Blood DNA methylation	2014-2015	Methylation of DNA from peripheral blood, LINE-1, Alu, Sat2