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Functional and Metabolic Effects of Ketone Bodies on Human Atrial Tissue in Patients With and Without Heart Failure

Not Applicable
Conditions
Heart Failure
Ischemia Reperfusion Injury
Mitochondrial Pathology
Interventions
Dietary Supplement: 3-hydroxybuturate
Registration Number
NCT04379934
Lead Sponsor
University of Aarhus
Brief Summary

This proof of concept study aims to evaluate the effects of applying ketone bodies to human atrial tissue biopsies using an atrial strips model and high resolution respirometry.

Detailed Description

Ketone bodies are high energy molecules which are naturally occurring in the body during fasting or stress.

Recently, ketone bodies have been shown to improve cardiac function in patients with heart failure(1) through a mechanism, which has yet to be elaborated.

We wish to evaluate the effects of ketone body administration on cardiac tissue subjected to ischemia reperfusion injury. We will evaluate contractile force of the and the mitochondrial performance. Cardiac atrial tissue will be obtained from patients undergoing coronary artery bypass grafting (CABG) or valve replacement surgery. Patients will be recruited after signing an informed consent and allocated in heart failure / non-heart failure groups based on a clinical evaluation.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
40
Inclusion Criteria
  • EF < / > 45%, hospitalized for elective CABG or heart valve surgery
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Exclusion Criteria
  • Myocardial infarction within 4 weeks, atrial fibrillation, medically treated thyroid disease, oral treatment with opioids.
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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Non-heart failure3-hydroxybuturateEjection fraction \> 45%
Heart failure3-hydroxybuturateEjection fraction \< 45%
Primary Outcome Measures
NameTimeMethod
Contractile force recoveryOutcome will be assessed within a year when the inclusion of patients is complete

Atrial strips model, contractile force (nm\*mm\^-1) during stabilization, ischemia and reperfusion

Secondary Outcome Measures
NameTimeMethod
Mitochondrial respiratory capacityOutcome will be assessed within a year when the inclusion of patients is complete

High Resolution Respirometry, Complex I and Complex I+II respiration (pmol O2 · s-1·mg-1) assessed via a substrate and inhibitor (SUIT) protocol.

Mitochondrial Reactive Oxygen Species productionOutcome will be assessed within a year when the inclusion of patients is complete

High Resolution FluoRespirometry, Complex I and Complex I+II linked ROS generation measured with AmplexUltra red and H2O2 interaction fluorescense

Trial Locations

Locations (1)

Aarhus University Hospital

🇩🇰

Aarhus, Denmark

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