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Berry Components and Blood Sugar & Fat Metabolism

Not Applicable
Completed
Conditions
Healthy Volunteers
Registration Number
NCT03458858
Lead Sponsor
USDA Beltsville Human Nutrition Research Center
Brief Summary

The primary objective of this study is to determine which components of berries improve glucose metabolism and increase fat oxidation in humans.

Detailed Description

The aim of this study is to improve understanding of how diet can regulate glucose metabolism and fuel use. Previous human and animal studies have demonstrated that berries can improve gluco-regulation and increase fat oxidation. The aim of this study is to determine which component of berries is conveying this health benefit.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
36
Inclusion Criteria
  • Healthy non-smoker
  • able to understand consent
Exclusion Criteria
  • Body Mass Index less than 25 kg/m2
  • pregnant, lactating, having given birth in the past year
  • allergy to berries
  • blood glucose greater than 125 mg/dL
  • history of bariatric surgery or nutrient malabsorption disease
  • diagnosis or treatment of cancer in past three years
  • type 2 diabetes requiring the use of diabetes pills, insulin, or non-insulin shots

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Primary Outcome Measures
NameTimeMethod
Glucose Metabolism Phase 1Day 8

Subjects will undergo a meal-based glucose tolerance test; blood samples will be analyzed for glucose in mmol/L.

Glucose Metabolism Phase 2Day 29

Subjects will undergo a meal-based glucose tolerance test; blood samples will be analyzed for glucose in mmol/L.

Glucose Metabolism Phase 3Day 50

Subjects will undergo a meal-based glucose tolerance test; blood samples will be analyzed for glucose in mmol/L.

Glucose Metabolism Phase 4Day 71

Subjects will undergo a meal-based glucose tolerance test; blood samples will be analyzed for glucose in mmol/L.

Secondary Outcome Measures
NameTimeMethod
Insulin sensitivityDay 8, 29, 50, 71

Subjects will undergo a meal-based glucose tolerance test; blood samples will be analyzed for insulin in units of microIU/mL.

Fat traffickingDay 8, 29, 50, 71

Subjects will undergo a meal-based glucose tolerance test; blood samples will be analyzed for non-esterified fatty acids in mmol/L.

Peripheral mononuclear blood cells energeticsDay 8, 29, 50, 71

Blood cells called peripheral mononuclear blood cells will be isolated and analyzed for mitochondrial function/oxygen consumption in nmol/ml.

Metabolic Fuel UseDay 7-8, 28-29, 49-50, 70-71

Subjects will spend the night in an indirect calorimeter to measure their oxygen consumption and carbon dioxide evolution which will be used to calculate the dietary energy source being oxidized, expressed as respiratory quotient which is a unitless ratio of liters of O2 inhaled to liters of CO2 exhaled.

Trial Locations

Locations (1)

USDA-ARS Beltsville Human Nutrition Research Center

🇺🇸

Beltsville, Maryland, United States

USDA-ARS Beltsville Human Nutrition Research Center
🇺🇸Beltsville, Maryland, United States

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