Dairy milk's potential as a functional food for improved cellular DNA integrity study

Recruiting

• Conditions: Integrity of genetic material in healthy volunteers; Public Health - Other public health

• Registration Number: ACTRN12611001147976
• Lead Sponsor: The Dairy Research Institute

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2011-11-02
• Last Update Posted: 13 January 2020

Recruitment & Eligibility

• Status: Recruiting
• Sex: All
• Target Recruitment: 16

Inclusion Criteria

Healthy subjects aged 20-40 years
Not undergoing treatment for life-threatening diseases
Not taking vitamin or micronutrient supplements at doses that exceed 25% of the recommended dietary intake.
Consume less that 250ml of milk per day
Consume less than 100g of semi-solid (e.g. yoghurt) or solid dairy foods (e.g. cheddar cheese) per day

Exclusion Criteria

Unable to attend an information session and/or read the information sheet
Females who are planning a pregnancy or are pregnant
Current smokers
Those who drink more than two standard drinks of alcohol per day.
Body Mass Index 30 or greater
Diagnosed with diabetes
Lactose intolerance

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Radiation induced micronuclei in lymphocytes using cytokinesis blocked micronucleus (CBMN) assay.[At each of the three visits at time points 0hr, 1.5hr, 3hr and 4.5hrs after drinking either of the three test drinks];Micronuclei in long term lymphocyte cultures using cytokinesis blocked micronucleus (CBMN) assay.[The plasma isolated from different points of the intervention will be used to detect its genome-protective effect in long-term (10 day) lymphocyte cultures using the micronucleus index in the CBMNcyt assay.]

Secondary Outcome Measures

Name	Time	Method
Plasma vitamin C will be measured using well established High Performance Liquid Chromatography (HPLC) method.[At each of the three visits at time points 0hr, 1.5hr, 3hr and 4.5hrs after drinking either of the three test drinks];Plasma calcium will be measured using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) methods.[At each of the three visits at time points 0hr, 1.5hr, 3hr and 4.5hrs after drinking either of the three test drinks]