Mitochondrial function of monocytes during prolonged hypobaric hypoxia and influence of antioxidants – a randomized controlled study
Not Applicable
- Conditions
- healthy voluteers subjected to hypobaric hypoxic conditions
- Registration Number
- DRKS00003242
- Lead Sponsor
- Inselspital Bern
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete
- Sex
- All
- Target Recruitment
- 12
Inclusion Criteria
adult, healthy, well-trained mountaineers with previous trekking experience and basic mountaineering skills
Exclusion Criteria
any type of cardiac, renal, hepatic or respiratory diseases or acute or chronic infectious or inflammatory disease, presence of any metabolic disorder, regular intake of medications, and the development of high-altitude pulmonary edema or high-altitude cerebral edema after rapid ascent to altitudes below 3500 m
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method changes in mitochondrial function of peripheral blood monocytes measured as activity of citrate synthase using a citrate synthase assay kit (Sigma-Aldrich, CS0720) and activity of cytochrome c oxidase using a cytochrome c oxidase assay kit (Sigma-Aldrich, CYTOCOX1) on the altitudes of 440m (baseline), 3700m (day 1), 4500m (day 4) and 5400m (day 9 ) of the expedition.
- Secondary Outcome Measures
Name Time Method