Mitochondrial Dysfunction and Insulin Resistance in Skeletal Muscle
概览
- 阶段
- 不适用
- 干预措施
- Intralipid, 20% Intravenous Emulsion
- 疾病 / 适应症
- Insulin Resistance
- 发起方
- University of Copenhagen
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Whole body Insulin sensitivity
- 状态
- 已完成
- 最后更新
- 4年前
概览
简要总结
The purpose of the study is to evaluate the link between insulin resistance and alterations in skeletal muscle mitochondrial redox homeostasis
研究者
Morten Hostrup, PhD
Associate Professor
University of Copenhagen
入排标准
入选标准
- •Body mass index (BMI) ≥25 and \<40 kg/m2
- •Fasting plasma glucose ≥ 5.6 mmol/L or HbA1c ≥ 5.7%
- •HOMA2-IR \> 1.4
- •VO2max \<45 ml/kg/min
排除标准
- •Treatment with \>2 antidiabetic medications
- •Insulin usage
- •Chronic disease deemed by the study responsible medical doctor to interfere with any part of the study
- •Chronic use of prescription medicine deemed by the study responsible medical doctor to interfere with any part of the study
研究组 & 干预措施
Lipid infusion + MitoQ
Subjects undergo a hyperinsulinemic isoglycemic clamp preceded by MitoQ administration and intravenous lipid infusion
干预措施: Intralipid, 20% Intravenous Emulsion
Lipid infusion + MitoQ
Subjects undergo a hyperinsulinemic isoglycemic clamp preceded by MitoQ administration and intravenous lipid infusion
干预措施: MitoQ
Lipid infusion + placebo
Subjects undergo a hyperinsulinemic isoglycemic clamp preceded by placebo administration and intravenous lipid infusion
干预措施: Intralipid, 20% Intravenous Emulsion
Lipid infusion + beta2-agonist
Subjects undergo a hyperinsulinemic isoglycemic clamp with intravenous infusion of lipid and salbutamol
干预措施: Intralipid, 20% Intravenous Emulsion
Lipid infusion + beta2-agonist
Subjects undergo a hyperinsulinemic isoglycemic clamp with intravenous infusion of lipid and salbutamol
干预措施: Salbutamol
结局指标
主要结局
Whole body Insulin sensitivity
时间窗: 5 hours after lipid infusion
Whole body insulin sensitivity is determined by hyperinsulinemic isoglycemic clamp method
Skeletal muscle insulin sensitivity
时间窗: 5 hours after lipid infusion
Insulin-dependent skeletal muscle glucose uptake is determined by hyperinsulinemic isoglycemic clamp method integrated with measurements of femoral artery blood flow and arteriovenous difference of glucose
次要结局
- Mitochondrial respiration(Baseline)
- Mitochondrial reactive oxygen species(Baseline)
- Mitochondrial oxidative stress(Before (baseline) as well as 3 and 5 hours after lipid infusion)
- Muscle redox status(Before (baseline) as well as 3 and 5 hours after lipid infusion)
- Insulin signalling(Before (baseline) as well as 3 and 5 hours after lipid infusion)