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A Novel Faecal Microbiota Transplantation System for Treatment of Primary and Recurrent Clostridium Difficile Infection

Not Applicable
Conditions
Clostridium Difficile Infection
Interventions
Biological: faecal human microbiota transplant (FMT)
Registration Number
NCT03053505
Lead Sponsor
Sejtterapia Kozpont Kft.
Brief Summary

This study is a two-arm, interventional, prospective, open-label, multi-center clinical trial with randomized and non-randomized study groups to evaluate the safety and effectiveness of faecal microbiota transplantation (FMT) for the treatment of adult patients suffering from primary or recurrent Clostridium difficile infection (CDI), using a novel, standardized microbiota transplantation system.

Detailed Description

Clostridium difficile is an anaerobe, spore-forming bacillus. Infections with its toxin-producing strains are capable of causing CD associated enteral disease ranging in severity from mild diarrhea to fatal fulminant colitis. CD infection(CDI) occurs among patients who have taken antibiotics previously, suggesting that the normal gut flora is capable of preventing CDI. The disease is mainly treated with antibiotics, however, these antibiotics show high therapeutic failure and recurrence rates. There is significant interest in the development of alternative therapeutic strategies. Among the alternative methods only faecal microbiota transplantation (FMT) is gaining acceptance due to its excellent cure rate and low recurrence rate. FMT is a new approach to treating CDI, since no further antibiotics are administered, instead the normal gut flora being restored by administering faecal homogenisate from a healthy donor. Immediate risks of FMT are minimal, its efficacy is excellent,but further data is required about its short and long term safety, its most appropriate timing during the course of CDI and the optimal technical protocol for preparing the fecal homogenisate. In addition, the procedure is also challenging and the intervention itself is unappealing in nature. To address the challenges described above a novel faecal transplantation system has been designed (Burgin-Matic System, BMS), which is suitable for the production of faecal bacterial suspension in a standardized and controlled environment. Using this new approach, a multi-center,prospective,interventional clinical study involving two groups of patients has been designed: 1. In a non-randomized group("R") the safety and efficacy of FMT with the new, automated transplantation system will be assessed on 50 patients suffering from "R"ecurrent CDI. 2. In a randomized group ("F") FMT will be compared with the gold standard vancomycin treatment for 2x50 patients, with their "F"irst episode of CDI, suffering from severe infection or at risk of developing recurrent or severe disease and not responding to at least 72 hours of antibiotic treatment. In the non-randomized group("R"), the safety and efficacy of FMT will be assessed,with the hypothesis that FMT with the BMS is equally safe and effective(non-inferior)as reported in the international studies. In the randomized group ("F") primary endpoints will be the clinical cure rate at various time points, global cure rate at 10 weeks, time to clinical cure and time to global cure, while as secondary endpoints the cost effectiveness, quality of life, mortality will be assessed also. Our hypothesis is, that FMT with the BMS is superior to vancomycin treatment in terms of primary and secondary endpoints for these patients.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
150
Inclusion Criteria
  • Group "R":- recurrent CDI;- positive stool toxin test within 72 hours before enrolment
  • Group "F":- first (initial) episode of CDI;- enrolled patient falls in at least one of the following categories:high risk of recurrence or high risk of developing severe CDI or severe or life-threatening CDI;- patient requires hospitalization or CDI occurs during a hospital stay;- persisting symptoms despite least 72 hours of adequate antibiotic treatment;-positive stool CD toxin test obtained within 72 hours before screening;- in all cases, primary consideration must be given to the severity and pace of the patient's CDI when deciding whether early use of FMT is appropriate to prevent further clinical deterioration.
Exclusion Criteria
  • absence of either patient's or its legally authorized representative's informed consent
  • inability or unwillingness to comply with protocol requirements
  • severe co-morbidities, terminal underlying disease with a life expectancy of less than 90 days
  • pregnancy or breastfeeding
  • active gastroenteritis caused by microorganisms other than CD
  • underlying chronic gastrointestinal disease that causes diarrhoea such as autonomic diabetic neuropathy, short bowel syndrome, faecal incontinence, active inflammatory bowel disease
  • alimentary or over-the-counter drog allergy with previous anaphylactic reaction
  • absolute contraindication to FMT

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Primary CDI FMTfaecal human microbiota transplant (FMT)Randomized group ("F" FMT) for the treatment of primary CDI with FMT
Recurrent CDI FMTfaecal human microbiota transplant (FMT)Non-randomized group ("R") for treatment of recurrent CDI with FMT
Primary CDI antibioticVancomycin or FidaxomicinRandomized group ("F" AB) for the treatment of primary CDI with antibiotics (vancomycin or fidaxomicin)
Primary Outcome Measures
NameTimeMethod
Global cure rate at 10 weeks10 weeks after enrolment
Time to clinical cureThrough study completion, an average of 18 months

The number of days between enrolment and the resolution of diarrhoea

Time to global cureThrough study completion, an average of 18 months

The number of days between enrolment and the resolution of diarrhoea without relapse

Cure rate at 2 weeks2 weeks after enrolment
Cure rate at 4 weeks4 weeks after enrolment
Treatment failure rateThrough study completion, an average of 18 months
Recurrence rate 8 weeks after clinical cure8 weeks after clinical cure
Secondary Outcome Measures
NameTimeMethod
Number of adverse events (AE)Through study completion, an average of 18 months

Number of participants with treatment related adverse events

Number of serious adverse events (SAE)Through study completion, an average of 18 months

Number of participants with treatment related serious adverse events

Time of hospitalizationThrough study completion, an average of 18 months
Days without diarrhoea during study periodThrough study completion, an average of 18 months
Patient related quality of life0, 7, 14 days after enrolment

Measured with EuroQoL 5Q-TL questionnaire

Professional acceptanceThrough study completion, an average of 18 months

A modified TSQM-14 questionnaire for assessing professional acceptance will be used during the study

General health survey for patients0, 7, 14 days after enrolment

Measured with SF-36v2 questionnaire

Patient anxiety and depression0, 14, 70 days after enrolment

Measured with HAD Scale (HADS)

Patient acceptance of treatment14,70 days after enrolment

Measure with TSQM-14 questionnaire

Trial Locations

Locations (3)

University of Debrecen, Clinical Centre

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Debrecen, Hajdu-Bihar megye, Hungary

Szabolcs-Szatmar-Bereg Megyei Korhazak es Egyetemi Oktatokorhaz

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Nyiregyhaza, Szabocs-Szatmar-Bereg megye, Hungary

Miskolci Semmelweis Korhaz es Egyetemi Oktatokorhaz

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Miskolc, B-A-Z County, Hungary

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