Exercise in Hypoxia and Nitrate Supplementation in Athletes: is the Whole Greater Than the Sum of Its Parts?

Not Applicable

Completed

• Conditions: Hypoxia

• Registration Number: NCT06727656
• Lead Sponsor: University of Trás-os-Montes and Alto Douro

Brief Summary

Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions.

Detailed Description

Determine the combined effect of training in hypoxia and nitrate supplementation on the performance and health of athletes at sea-level and altitude conditions. It is hypothesized that the combined effect of these interventions will promote an additional performance and health improvement in this population at sea-level and altitude conditions, compared with the single effect.

Study Timeline

• Study Start: 2019-02-01
• Primary Completion: 2020-07-22
• Study Completion: 2020-07-22
• Study First Submitted: 2024-07-24
• Status Verified: 2024-12
• Study First Submitted QC: 2024-12-04
• Last Update Submitted: 2024-12-04
• Study First Posted: 2024-12-11
• Last Update Posted: 2024-12-11

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 30

Inclusion Criteria

• trained male subjects, regularly involved in competitive running, cycling and/ or triathlon events (4-5 times per week) in the past five years

Exclusion Criteria

• smoking or other chronic issues
• using medication or dietary supplements in the last 3 months, or during the duration of the study
• being exposed to altitude (≥2000 m) in the last 3 months

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Primary Outcome Measures

Name	Time	Method
Performance adaptations	Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)	VO2 kinetics (time constant and amplitude of fast and slow oxygen components)
Cardiovascular and neuromuscular adaptations	Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)	Muscular oxygen saturation (SmO2)
Skeletal muscle adaptations	Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)	Mitochondrial and capillary density, capillary-to-fibre ratio and fibre cross-section area - cryosections cut with a cryostat and further samples analysed by fluorescence microscopy
Haematological adaptations	Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)	Plasma \[NO2-\] and \[NO3-\], erythropoietin (EPO), haemoglobin (Hb) and haematocrit (Hct) - all assays on plasma sample analysed by spectrophometry
Oxidative stress and antioxidant markers adaptations	Moment #1 (48hours pre-intervention) and moment #2 (48hours post-intervention)	Nitrotyrosine, malondialdehyde, ferric-reducing antioxidant power and superoxide dismutase concentrations - all assays on plasma sample analysed by spectrophometry

Secondary Outcome Measures

Name	Time	Method
Sleep and cardiac autonomic activity adaptations	Moment #1 (2 weeks pre-intervention - baseline) and moment #2 (4 weeks intervention)	Heart rate variability (lnRMSSD)

Trial Locations

Locations (1)

University of Trás-os-Montes e Alto-Douro; 🇵🇹 Vila Real, Portugal