Effect of nut consumption on semen quality and functionality in healthy males

Not Applicable

Completed

• Conditions: Semen quality and functionality; Not Applicable

• Registration Number: ISRCTN12857940
• Lead Sponsor: INC International Nut and Dried Fruit Council Foundation (Spain)

Brief Summary

1. 2018 results in: https://www.ncbi.nlm.nih.gov/pubmed/30475967 2. 2019 results in: https://www.ncbi.nlm.nih.gov/pubmed/30833599 3. 2019 results in: https://doi.org/10.3390/nu11061372 (added 20/06/2019)

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2016-05-25
• Study Completion: 2017-08-31
• Last Update Posted: 15 July 2019

Recruitment & Eligibility

• Status: Completed
• Sex: Male
• Target Recruitment: 119

Inclusion Criteria

1. Healthy males
2. 18-35 years old
3. Reported a western-style diet

Exclusion Criteria

1. Frequent consumption of nuts or a known history of allergy
2. Use of plant sterol or fish oil supplements and multivitamins, vitamin E or other antioxidant supplements
3. History of reproductive disorders or vasectomy
4. Current smokers
5. Taking antioxidant supplements or medications for chronic illness
6. Use of illegal drugs

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Improvement of sperm vitality and motility. Semen samples will be collected at baseline and the end of the intervention period. All men will be instructed to abstain for 3 days prior to providing the study specimen, with abstinence period verified by self-report on day of sample collection. Semen samples will be collected allowing analysis within 60 min of sample production. A single researcher, who was blinded as to intervention versus control group status, will perform the conventional semen analyses. For the rest of analysis aliquots of semen will be frozen at -196ºC until analysis.

Secondary Outcome Measures

Name	Time	Method
<br> Semen samples will be collected at baseline and the end of the intervention period:<br> 1. Other conventional semen parameters<br> 2. Plasma lipid profile, glucose and insulin concentrations<br> 3. Serum folic acid and alpha-linolenic concentrations<br> 4. Sperm DNA fragmentation<br> 5. Sperm ROS evaluation<br> 6. Sperm miRNA expression profiles<br> 7. Sperm DNA methylation<br> 8. Sperm chromosome stability<br>