Mitochondrial Capacity in Young and Elderly Males

Completed

Conditions: Sarcopenia

Registration Number: NL-OMON22045

Lead Sponsor: WO, TIF

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 40

Inclusion Criteria

Young
•Male, 19-25 years
•BMI 18.5-27 kg/m2
•Normal activity levels: 1-2 hour per week engaged in a structured exercise session

Elderly
•Male, 65-71 years
•BMI 18.5-27 kg/m2
•Normal activity levels: 1-2 hour per week engaged in a structured exercise session
•Short physical performance battery score >6

Exclusion Criteria

•(known symptoms of) Metabolic diseases, e.g. type I or II diabetes,
•Health concerns regarding respiratory and pulmonary diseases, e.g. COPD, (exercise induced) asthma and cardiovascular disease.
•Impaired physical performance (defined as short physical performance battery score < 6) (Only for elderly group)
•Contraindications for electrical stimulation, e.g. cardiac pacemaker
•Contraindications for muscle biopsy, e.g. usage of anticoagulants (low dose aspirin allowed)
•Regular smoker (defined as smoking >5 cigarettes per week)
•Vastus lateralis skinfold >30mm
•Haemoglobin concentrations below 8.0 mmol/L
•Usage of recreational drugs, e.g. marihuana, amphetamines and cocaine during the study (starting after first screening day)
•Recent usage (within four months) of supplements with suggestive training effects, e.g. creatine phosphate, EPO or anabolic steroids.
•Current or recent (<2 weeks) participation in other clinical trials

Study & Design

Study Type: Observational non invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
The main study parameter is mitochondrial capacity, measured as the rate of recovery of muscle oxygen consumption after exercise measured using NIRS in the vastus lateralis, gastrocnemius and tibialis anterior.

Secondary Outcome Measures

Name	Time	Method
Physical activity is monitored using a wearable accelerometer over a period of seven days and a 24 hour urine is collected in order to analyse markers of mitochondrial function in urine. Blood is collected to analyse markers of mitochondrial function in plasma.