The long-term effect of 10 days cold in diabetic patients.
Completed
- Conditions
- Cold acclimationNon insulin depended diabetes mellitusInsulin sensitivityObesitas (BMI 28-35)Cardiovascular risksLipid metabolismInsulinegevoeligheidKoude acclimatieOvergewichtDiabetes mellitus type 2Vetmetabolismecardiovasculair risico
- Registration Number
- NL-OMON22285
- Lead Sponsor
- Maastricht university (NUTRIM)
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 16
Inclusion Criteria
Caucasians
- Age: 45 – 70 years
Exclusion Criteria
- Participate in physical activity more than 2x/week
- Unstable body weight (weight gain or loss > 5 kg in the last three months)
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method - Insulin sensitivity as determined by hyperinsulinemic euglycemic clamp. <br /><br>- Metabolism of triglyceride, cholesterol and lipoproteins after a meal.<br>
- Secondary Outcome Measures
Name Time Method - Endothelial dependent and independent vasodilation (measured with Laser Doppler Flowmetry and iontophoresis)<br /><br>- Arterial stiffness (measured with Pulse Wave Velocity)<br /><br>- Central retinal arterial equivalent (CRAE), central retinal vein equivalent (CRVE) and arteriovenous ratio (AVR)<br /><br>- Muscle biopsy: analysis of insulin signalling and insulin independent pathway causing GLUT4 translocation. Analysis of other relevant markers of energy metabolism. Cell culture to investigate the underlying molecular pathway. Skeletal muscle lipid accumulation (measured by immunofluorescence microscopy).<br /><br>- Intrahepatic lipid content (measured with 1H-MRS)<br /><br>- Skin temperature during cold exposure<br /><br>- HbA1c, endothelial/inflammatory markers and energy metabolism markers<br /><br>- Thermal sensation and comfort<br>