The Effect of Meat and Food Drived Polyphenols on Oxidize LDL Level

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Other: frozen meat cutlets meal; Other: meat meals

• Registration Number: NCT01389492
• Lead Sponsor: Hadassah Medical Organization

Brief Summary

The postprandial increase in MDA level following meat meal could cause modification to LDL particles in healthy volunteers. Co-consumption of food drived polyphenols with the meat will result in a significant decrease in LDL modification.

Detailed Description

Not available

Study Timeline

• Status Verified: 2009-10
• Study Start: 2010-10
• Primary Completion: 2011-02
• Study Completion: 2011-05
• Study First Submitted: 2011-07-03
• Study First Submitted QC: 2011-07-06
• Last Update Submitted: 2011-07-06
• Study First Posted: 2011-07-08
• Last Update Posted: 2011-07-08

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 14

Inclusion Criteria

• Healthy adults, normal BMI

Exclusion Criteria

• Metabolic disorders
• Smokers
• Drinkers
• Taking nutritional supplement

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
fresh meat	frozen meat cutlets meal	250 g of fresh meat meal
fresh meat	meat meals	250 g of fresh meat meal
fresh meat and wine	frozen meat cutlets meal	250 g of fresh meat meal
frozen meat	frozen meat cutlets meal	250 g of frozen meat meal for 4 days
frozen meat	meat meals	250 g of frozen meat meal for 4 days
frozen meat and wine	frozen meat cutlets meal	250 g of frozen meat and red wine for 4 days
frozen meat and wine	meat meals	250 g of frozen meat and red wine for 4 days
fresh meat and wine	meat meals	250 g of fresh meat meal

Primary Outcome Measures

Name	Time	Method
Change in plasma MDA levels following meat meal	Fasting before meal and 3, and 6 h postprandial, and fasting levels	After an overnight fast baseline (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The subjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 and 6 h after the meal was eaten. Serum was separated from whole blood by centrifugation (910 g, 15 min), and MDA was evaluated by HPLC in plasma samples
Changes in MDA concentration on LDL fraction following meat meals	Fasting, before meal was eaten, 3 h following meal, at day 4, 3 h following meal consumption, and day 5 following over nigth fast	Subject consumed the test meals for 4 days. Day 1, (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The sbjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 h after the meal was eaten. Blood was collected at day 4, 3 h after the meal was eaten, at the morning of day 5.Plasma LDL was isolated using a single discontinuous density gradient ultracentrifugation. The protein concentration was determined based on the method of Bradford. MDA concentration was evaluated by HPLC.

Trial Locations

Locations (1)

The Hebrew University of Jerusalem; 🇮🇱 Jerusalem, Israel