Microvascular flow and microvascular blood volume measurements in human skeletal muscle using contrast enhanced ultrasound (CEUS).

Completed

Conditions: microvascular insulin resistance
obesity
10018424
10057166

Registration Number: NL-OMON38401

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 8

Inclusion Criteria

1. Age 18-55 years
2. BMI < 25 kg/m2 *;* = most likely to have intact capillary recruitment in skeletal muscle (a parameter studied in this research protocol).

Exclusion Criteria

1. Cardiovascular disease (stroke, coronary artery disease, peripheral vascular disease, heart failure, cardiac shunts, heart valve replacement, pulmonary hypertension).
2. Pulmonary disease.
3. Diabetes mellitus (FPG > 7.8 mmol/L).
4. Smoking.
5. Hypertension (>140/90 mmHg).
6. Alcohol use > 4 U/day.
7. Use of medication (antihypertensive medication, statins, corticosteroids, NSAIDs, ciclosporin A, rifampicin).
8. Pregnancy or lactation.
9. Insufficient knowledge of the Dutch language.
10. Known allergic reaction to ultrasound contrast-agent.
11. Known cardiac dysrhythmias (e.g. atrial fibrillation, long QT-syndrome) or a family history of cardiac dysrhythmias or sudden cardiac death.

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>1. Intra-observer variability and day-to-day variation of forearm skeletal<br /><br>muscle CEUS measurements.<br /><br>2. The percentage change in microvascular blood volume (MBV), flow and flow<br /><br>velocity (MFV), measured by means of CEUS signal, in forearm skeletal muscle<br /><br>from baseline to post-exercise status.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>1. Inter-observer variability in offline analysis of CEUS measurements.<br /><br>2. Skeletal muscle microvascular flow distribution.<br /><br>3. Change in skeletal muscle microvascular flow distribution from baseline to<br /><br>post-exercise status.</p><br>