The role of surgery- induced inflammatory mediators in macrophage activatio

Completed

• Conditions: colon cancer; Colorectal carcinoma; 10017990; 10017991; 10017998

• Registration Number: NL-OMON39271
• Lead Sponsor: Vrije Universiteit Medisch Centrum

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 29 April 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 9

Inclusion Criteria

Resection of primary colorectal carcinoma.

Exclusion Criteria

Excisting infection: Crohn's disease or ulcerative colitis

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>- Capacity of pre- per- and post operative plasma to activate macrophages in<br /><br>vitro. Endpoints are production of reactive oxygen species (ROS) and conversion<br /><br>of MTT to formazan (this allows to assess the viability of cells).<br /><br><br /><br>- Identification of the inflammatory factors released after surgery that are<br /><br>responsible for macrophage activation. Endpoints are decrease of ROS production<br /><br>and conversion of MTT in the presence of blocking agents against inflammatory<br /><br>mediators.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>not applicable</p><br>