eutrophil extracellular trap induced changes in (RSV-infected) pediatric human airway epithelial (HAE) cultures.

Withdrawn

Conditions: respiratoir syncytieel virus bronchiolitis
RSV-bronchiolitis
10047438
10024970

Registration Number: NL-OMON49719

Lead Sponsor: Academisch Medisch Centrum

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Withdrawn

Sex: Not specified

Target Recruitment: 20

Inclusion Criteria

- Age between 2 and 24 months
- No known pulmonary disease
- Endotracheal intubation (for elective surgery)
- No signs of infection (fever, temperature >38.5 C) and no respiratory
symptoms (cough, wheeze, snotty) present at moment of intubation.
- No reported respiratory symptoms in the last 7 days prior to surgery (cough,
wheeze, snotty)
- Informed consent obtained for both non-endoscopic cytobrushing and
serological testing after confirmation of comprehension of the Dutch language.

Exclusion Criteria

- Age < 2 months or > 24 months
- Reported pulmonary disorder
- Non-elective endotracheally intubation
- Symptoms of a present or recent infection (T>38.5C in the last 7 days prior
to surgery) or reported respiratory symptoms in the last 7 days prior to
surgery
- No consent.

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>We will use the collected cells to obtain pediatric HAE cultures. We will use<br /><br>these cultures as an ex vivo model to study NETs induced changes in the context<br /><br>of RSV infection of the pediatric airways. We will perform measurements on<br /><br>airway mucus (protein expression) and airway epithelial damage (necrosis,<br /><br>apoptosis, loss of differentiation, loss of barrier function, apoptotic gene<br /><br>expression, inflammation markers and inflammatory pathways). </p><br>

Secondary Outcome Measures