Effect of Spinal or Sevoflurane Anesthesia on Neutrophil Activation During Knee Surgery With Pneumatic Tourniquet

Not Applicable

Completed

• Conditions: Knee Surgery With Tourniquet
• Interventions: Drug: Bupivacaine / SUFentanil; Drug: Sevoflurane Volatile Liquid

• Registration Number: NCT03470363
• Lead Sponsor: University of Liege

Brief Summary

Aim of study : Assessement of neutrophil activation during ischemia reperfusion associated with tourniquet use for total knee arthroplasty. This activation was compared in a group of patient scheduled for spinal anesthesia and for sevoflurane anesthesia

Detailed Description

Aim of study : Assessement of neutrophil activation during ischemia reperfusion associated with tourniquet use for total knee arthroplasty. This activation was compared in a group of patient scheduled for spinal anesthesia and for sevoflurane anesthesia This activation was assessed by plasma myeloperoxidase and elastase measurements in four consecutive blood samples during and after surgery (before surgical incision and 1, 3, 24 hours following tourniquet release.

Study Timeline

• Study Start: 2016-01-01
• Primary Completion: 2018-03-01
• Study Completion: 2018-03-01
• Study First Submitted: 2018-03-12
• Study First Submitted QC: 2018-03-16
• Study First Posted: 2018-03-19
• Status Verified: 2020-03
• Last Update Submitted: 2020-03-24
• Last Update Posted: 2020-03-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 40

Inclusion Criteria

• Total knee arthroplasty under spinal or sevoflurane anesthesia

Exclusion Criteria

• American Society of Anesthesiologists class 3 and 4
• vascular disease of the lower limbs
• cardiac or respiratory disease
• immune disorder

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Group Spinal anesthesia	Bupivacaine / SUFentanil	Knee surgery under spinal anesthesia Intervention involves spinal administration of 12,5 mg bupivacaine and 2,5 microgram sufentanil.
Group Sevoflurane anesthesia	Sevoflurane Volatile Liquid	Knee surgery under sevoflurane anesthesia Intervention involves administration of inhaled sevoflurane for maintenance of general anesthesia

Primary Outcome Measures

Name	Time	Method
Changes in plasmatic concentration of myeloperoxidase and elastase	Before surgical incision then 3 and 24 hours after tourniquet release	Biological measurement of changes in plasmatic concentration of myeloperoxidase and elastase from preoperative values to 3 and 24 hours after tourniquet release.

Secondary Outcome Measures

Name	Time	Method
Changes in plasmatic concentration of endothelial biomarkers	Before surgical incision then 3 and 24 hours after tourniquet release	Biological measurement of changes in plasmatic concentration of syndecan-1, soluble thrombomodulin, soluble E-selectin and VEGF from preoperative values to 3 and 24 hours after tourniquet release.