Collagen supplementation for tendon health

Not Applicable

• Conditions: Tendon protein synthesis in otherwise healthy males and females scheduled for anterior cruciate ligament (ACL) surgery.; Injury, Occupational Diseases, Poisoning

• Registration Number: ISRCTN72255101
• Lead Sponsor: HAN University of Applied Sciences

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2023-10-06
• Last Update Posted: 11 March 2024
• Study Completion: 2025-07-01

Recruitment & Eligibility

• Status: Ongoing
• Sex: All
• Target Recruitment: 30

Inclusion Criteria

1. Otherwise healthy male and female volunteers
2. Aged 18 to 40 years old
3. Recruited from patients attending the Department of Orthopedics from the participating hospital
4. Planned for ACL reconstruction with hamstring autograft
5. BMI =18.5 and =27.5 kg/m2

Exclusion Criteria

1. Blood donation during the study period or during the last 2 months
2. Pregnancy
3. Third-generation oral contraceptives
4. Currently smoking
5. Consumption of >21 alcoholic beverages per week
6. Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications)
7. Regular use of protein or other nutritional supplements (including vitamins).
8. Reported slimming or medically prescribed diet
9. Use of antibiotics in the past month
10. Current participation in another biomedical research study

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Tendon tissue protein synthesis rate, expressed as fractional synthesis rate (FSR, %/d), will be measured for 7 days prior to surgery. To calculate FSR, the standard precursor-product method will be used. Tendon protein synthesis rates will be calculated from:<br>1. Saliva 2H enrichment (i.e., precursor)<br>2. Tissue protein-bound enrichment 2H-alanine<br>3. Plasma albumin enrichment 2H-alanine<br>

Secondary Outcome Measures

Name	Time	Method
A ligament in muscle protein synthesis rate, expressed as fractional synthesis rate (FSR, %/d), will be measured for 7 days prior to surgery. To calculate FSR, the standard precursor-product method will be used. Tendon protein synthesis rates will be calculated from:<br>1. Saliva 2H enrichment (i.e., precursor)<br>2. Tissue protein-bound enrichment 2H-alanine<br>3. Plasma albumin enrichment 2H-alanine<br>